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wrap;">亞克隆</strong>就是初步克隆的外源片段往往較長，含有許多目的基因片段以外的DNA片段，將目的基因所對應的一小段DNA找出來。</div><div>對已經(jīng)獲得的目的DNA片段進行重新克隆，其目的在于對目的DNA進行進一步分析，或者進行重組改造等。<br type="_moz"/></div><div>基本過程包括：</div><div>(1)目的DNA片段和載體的制備；</div><div>(2)目的DNA片段和載體的連接；</div><div>(3)連接產(chǎn)物的轉(zhuǎn)化；</div><div>(4)重組子篩選。<br type="_moz"/></div><div>技術：限制性內(nèi)切酶是一類能識別雙鏈DNA分子中特異核苷酸序列的DNA水解酶，該類酶是體外剪切基因片段的重要工具。</div></div> <div class="sbrjztcv42p" id="nr_textbox"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="sbrjztcv42p" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="sbrjztcv42p" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="亞克隆" placeholder="請輸入產(chǎn)品名稱" /></div> </li> <li> <h4>您的單位：</h4> <div id="sbrjztcv42p" 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